Reduction of EGFR expression in SPC-A1 xenografts upon LPEI/siRNA-EGFR complexes injection, compared with control groups. (A) EGFR mRNA quantitative evaluation by real-time RT-PCR at 24 hours after injection. (B) Western Blot analysis, and (C) IHC staining of EGFR protein determination at 48 hours after injection. EGFR seen as brown stain, with magnification ×200 (upper) and ×400 (lower)(n = 6).All the results are shown as the mean±standard error of numbers obtained from 6 animals in each group. *P < 0.05.