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Table 6 Percent positive cells by FACS within BrdU + and BrdU- population in lung for pluripotent and pulmonary lineage specific markers

From: Looking for the elusive lung stem cell niche

 

BrdU-

TTF-1+

Oct3/4+

SSEA-3+

SSEA-4+

Sca-1+

Lin-

CD34+

CD31+

SP-C+

AQP-5+

CC-10

CD45+

CD45-

SP-C + CC10+

VEGF

Con 2d

92.13 ± 0.84

0.38 ± 0.10

0.560.09

0.55 ± 0.02

0.47 ± 0.14

0.22 ± 0.15

0.2 ± 0.01

0.31 ± 0.06

0.03 ± 0.01

5.59 ± 0.11

90.92 ± 0.50

1.30 ± 0.18

2.37 ± 0.17

83.70 ± 1.66

0.03 ± 0.01

2.86 ± 0.09

Con 6d

92.95±

0.31 ± 0.07

0.35 ± 0.02

0.4 ± 0.07

0.32 ± 0.03

0.06 ± 0.02

0.19 ± 0.04

0.22 ± 0.009

0.22 ± 0.09

4.90 ± 0.14

85.75 ± 2.21

1.33 ± 0.21

2.7 ± 0.16

76.15 ± 1.69

0.12 ± 0.03

2.31 ± 0.14

Bleo 2d

89.06 ± 0.66

11.36 ± 0.59

1.11 ± 0.09

0.28 ± 0.07

0.35 ± 0.02

0.12 ± 0.07

0.34 ± 0.03

3.45 ± 0.09

0.71 ± 0.042

1.48 ± 0.17

9.29 ± 0.41

2.60 ± 0.16

0.78 ± 0.06

74.65 ± 1.37

0.01 ± 0.004

12.74 ± 0.59

Bleo 4d

89.43 ± 0.66

12.13 ± 0.74

1.34 ± 0.09

0.32 ± 0.04

0.26 ± 0.08

0.25 ± 0.03

0.24 ± 0.08

3.42 ± 0.23

0.8 ± 0.09

1.74 ± 0.03

217.49 ± 208.50

2.85 ± 0.08

0.65 ± 0.13

74.65 ± 1.37

0.02 ± 0.004

12.45 ± 0.84

Bleo 6d

97.40 ± 1.1

11.68 ± 0.6

1.742 ± 0.16

0.31 ± 0.04

0.32 ± 0.01

0.16 ± 0.05

0.54 ± 0.05

2.90 ± 0.06

0.6 ± 0.086

1.52 ± 0.06

9.05 ± 0.18

2.36 ± 0.19

0.81 ± 0.13

83.70 ± 1.66

0.12 ± 0.06

10.65 ± 0.26

  1. BrdU + cells were gated and inside (positive and outside of the gate, BrdU negative) cells were characterized by FACS with fluorochrome conjugated monoclonal antibodies markers of pluripotence (TTF-1, SSEA-3,4, Oct ¾, SCA-1, Lineage negative and CD31 (endothelial progenitor) and CD34 (hematopoietic progenitor). lung lineages are characterized by the following markers: AQP-5+ cells are AEI, SP-C + cells are AEII and CC-10+ cells are Clara cells while SP-c + CC-10+ cells are broncho-alveolar stem cells.