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Table 6 Percent positive cells by FACS within BrdU + and BrdU- population in lung for pluripotent and pulmonary lineage specific markers

From: Looking for the elusive lung stem cell niche

  BrdU- TTF-1+ Oct3/4+ SSEA-3+ SSEA-4+ Sca-1+ Lin- CD34+ CD31+ SP-C+ AQP-5+ CC-10 CD45+ CD45- SP-C + CC10+ VEGF
Con 2d 92.13 ± 0.84 0.38 ± 0.10 0.560.09 0.55 ± 0.02 0.47 ± 0.14 0.22 ± 0.15 0.2 ± 0.01 0.31 ± 0.06 0.03 ± 0.01 5.59 ± 0.11 90.92 ± 0.50 1.30 ± 0.18 2.37 ± 0.17 83.70 ± 1.66 0.03 ± 0.01 2.86 ± 0.09
Con 6d 92.95± 0.31 ± 0.07 0.35 ± 0.02 0.4 ± 0.07 0.32 ± 0.03 0.06 ± 0.02 0.19 ± 0.04 0.22 ± 0.009 0.22 ± 0.09 4.90 ± 0.14 85.75 ± 2.21 1.33 ± 0.21 2.7 ± 0.16 76.15 ± 1.69 0.12 ± 0.03 2.31 ± 0.14
Bleo 2d 89.06 ± 0.66 11.36 ± 0.59 1.11 ± 0.09 0.28 ± 0.07 0.35 ± 0.02 0.12 ± 0.07 0.34 ± 0.03 3.45 ± 0.09 0.71 ± 0.042 1.48 ± 0.17 9.29 ± 0.41 2.60 ± 0.16 0.78 ± 0.06 74.65 ± 1.37 0.01 ± 0.004 12.74 ± 0.59
Bleo 4d 89.43 ± 0.66 12.13 ± 0.74 1.34 ± 0.09 0.32 ± 0.04 0.26 ± 0.08 0.25 ± 0.03 0.24 ± 0.08 3.42 ± 0.23 0.8 ± 0.09 1.74 ± 0.03 217.49 ± 208.50 2.85 ± 0.08 0.65 ± 0.13 74.65 ± 1.37 0.02 ± 0.004 12.45 ± 0.84
Bleo 6d 97.40 ± 1.1 11.68 ± 0.6 1.742 ± 0.16 0.31 ± 0.04 0.32 ± 0.01 0.16 ± 0.05 0.54 ± 0.05 2.90 ± 0.06 0.6 ± 0.086 1.52 ± 0.06 9.05 ± 0.18 2.36 ± 0.19 0.81 ± 0.13 83.70 ± 1.66 0.12 ± 0.06 10.65 ± 0.26
  1. BrdU + cells were gated and inside (positive and outside of the gate, BrdU negative) cells were characterized by FACS with fluorochrome conjugated monoclonal antibodies markers of pluripotence (TTF-1, SSEA-3,4, Oct ¾, SCA-1, Lineage negative and CD31 (endothelial progenitor) and CD34 (hematopoietic progenitor). lung lineages are characterized by the following markers: AQP-5+ cells are AEI, SP-C + cells are AEII and CC-10+ cells are Clara cells while SP-c + CC-10+ cells are broncho-alveolar stem cells.